文章基本信息

标题：Study on HPLC Fingerprint of Clematis manshurica Rupr from Different Areas
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作者：Juan Kong ; Xulong Huang ; Xiaosong Yang 等
期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2019
卷号：267
期号：6
页码：1-8
DOI：10.1088/1755-1315/267/6/062053
出版社：IOP Publishing
摘要：Objective: To establish HPLC fingerprint of Clematis manshurica Rupr from different areas. Method: 10 batches of Clematis manshurica Rupr was analyzed by RP-HPLC that the methods were used Odyssil-C₁₈ chromatographic column, with the mobile phase of acetonitril-0.1% phosphoric acid solution, the flow rate was 0.8mL/min, the wavelength was 210 nm, and column temperature was 30°C. And the similarity was evaluated by similarity evaluation software. Result: The HPLC-UV fingerprint of Clematis manshurica Rupr was established, and 20 common peaks were determined, the similarity was between 0.974-0.823, indicating that the differences of Clematis manshurica Rupr from different regions were significant. Conclusion: The analytical method has good separation degree and feasibility, and it can provide a reliable basis for the quality control of Clematis manshurica Rupr.