文章基本信息

标题：Molecular differentiation of five Cinnamomum camphora chemotypes using desorption atmospheric pressure chemical ionization mass spectrometry of raw leaves
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作者：Xiali Guo ; Meng Cui ; Min Deng 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2017
卷号：7
期号：1
DOI：10.1038/srep46579
语种：English
出版社：Springer Nature
摘要：Five chemotypes, the isoborneol-type, camphora-type, cineole-type, linalool-type and borneol-type of Cinnamomum camphora (L.) Presl have been identified at the molecular level based on the multivariate analysis of mass spectral fingerprints recorded from a total of 750 raw leaf samples (i.e., 150 leaves equally collected for each chemotype) using desorption atmospheric pressure chemical ionization mass spectrometry (DAPCI-MS). Both volatile and semi-volatile metabolites of the fresh leaves of C. camphora were simultaneously detected by DAPCI-MS without any sample pretreatment, reducing the analysis time from half a day using conventional methods (e.g., GC-MS) down to 30 s. The pattern recognition results obtained using principal component analysis (PCA) was cross-checked by cluster analysis (CA), showing that the difference visualized by the DAPCI-MS spectral fingerprints was validated with 100% accuracy. The study demonstrates that DAPCI-MS meets the challenging requirements for accurate differentiation of all the five chemotypes of C. camphora leaves, motivating more advanced application of DAPCI-MS in plant science and forestry studies.