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标题：The human two-pore channel 1 is modulated by cytosolic and luminal calcium
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作者：Laura Lagostena ; Margherita Festa ; Michael Pusch 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2017
卷号：7
期号：1
DOI：10.1038/srep43900
语种：English
出版社：Springer Nature
摘要：Two-pore channels (TPC) are intracellular endo-lysosomal proteins with only recently emerging roles in organellar signalling and involvement in severe human diseases. Here, we investigated the functional properties of human TPC1 expressed in TPC-free vacuoles from Arabidopsis thaliana cells. Large (20 pA/pF) TPC1 currents were elicited by cytosolic addition of the phosphoinositide phosphatidylinositol-(3,5)-bisphosphate (PI(3,5)P2) with an apparent binding constant of ~15 nM. The channel is voltage-dependent, activating at positive potentials with single exponential kinetics and currents are Na(+) selective, with measurable but low permeability to Ca(2+). Cytosolic Ca(2+) modulated hTPC1 in dual way: low μM cytosolic Ca(2+) increased activity by shifting the open probability towards negative voltages and by accelerating the time course of activation. This mechanism was well-described by an allosteric model. Higher levels of cytosolic Ca(2+) induced a voltage-dependent decrease of the currents compatible with Ca(2+) binding in the permeation pore. Conversely, an increase in luminal Ca(2+) decreased hTPC1 activity. Our data point to a process in which Ca(2+) permeation in hTPC1 has a positive feedback on channel activity while Na(+) acts as a negative regulator. We speculate that the peculiar Ca(2+) and Na(+) dependence are key for the physiological roles of the channel in organellar homeostasis and signalling.