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标题：Improved MALDI imaging MS analysis of phospholipids using graphene oxide as new matrix
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作者：Zhongjie Wang ; Yan Cai ; Yi Wang 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2017
卷号：7
期号：1
DOI：10.1038/srep44466
语种：English
出版社：Springer Nature
摘要：Matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is an increasingly important technique for detection and spatial localization of phospholipids on tissue. Due to the high abundance and being easy-to-ionize of phosphatidylcholine (PC), therefore, selecting matrix to yield signals of other lipids has become the most crucial factor for a successful MALDI-IMS analysis of phospholipids. Herein, graphene oxide (GO) was proposed as a new matrix to selectively enhance the detection of other types of phospholipids that are frequently suppressed by the presence of PC in positive mode. Compared to the commonly used matrix DHB, GO matrix significantly improved signal-to-noise ratios of phospholipids as a result of its high desorption/ionization efficiency for nonpolar compounds. Also, GO afforded homogeneous crystallizations with analytes due to its monolayer structure and good dispersion, resulting in better reproducibility of shot-to-shot (CV < 13%) and spot-to-spot (CV < 14%) analysis. Finally, GO matrix was successfully applied to simultaneous imaging of PC, PE, PS and glycosphingolipid in the mouse brain, with a total of 65 phospholipids identified.