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标题：Gfi1Cre mice have early onset progressive hearing loss and induce recombination in numerous inner ear non-hair cells
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作者：Maggie Matern ; Sarath Vijayakumar ; Zachary Margulies 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2017
卷号：7
期号：1
DOI：10.1038/srep42079
语种：English
出版社：Springer Nature
摘要：Studies of developmental and functional biology largely rely on conditional expression of genes in a cell type-specific manner. Therefore, the importance of specificity and lack of inherent phenotypes for Cre-driver animals cannot be overemphasized. The Gfi1(Cre) mouse is commonly used for conditional hair cell-specific gene deletion/reporter gene activation in the inner ear. Here, using immunofluorescence and flow cytometry, we show that the Gfi1(Cre) mice produce a pattern of recombination that is not strictly limited to hair cells within the inner ear. We observe a broad expression of Cre recombinase in the Gfi1(Cre) mouse neonatal inner ear, primarily in inner ear resident macrophages, which outnumber the hair cells. We further show that heterozygous Gfi1(Cre) mice exhibit an early onset progressive hearing loss as compared with their wild-type littermates. Importantly, vestibular function remains intact in heterozygotes up to 10 months, the latest time point tested. Finally, we detect minor, but statistically significant, changes in expression of hair cell-enriched transcripts in the Gfi1(Cre) heterozygous mice cochleae compared with their wild-type littermate controls. Given the broad use of the Gfi1(Cre) mice, both for gene deletion and reporter gene activation, these data are significant and necessary for proper planning and interpretation of experiments.