文章基本信息

标题：XTEND: Extending the depth of field in cryo soft X-ray tomography
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作者：Joaquín Otón ; Eva Pereiro ; José J. Conesa 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2017
卷号：7
期号：1
DOI：10.1038/srep45808
语种：English
出版社：Springer Nature
摘要：We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters.