文章基本信息

标题：A Raf-like protein kinase BHP mediates blue light-dependent stomatal opening
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作者：Maki Hayashi ; Shin-ichiro Inoue ; Yoshihisa Ueno 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2017
卷号：7
期号：1
DOI：10.1038/srep45586
语种：English
出版社：Springer Nature
摘要：Stomata in the plant epidermis open in response to blue light and affect photosynthesis and plant growth by regulating CO2 uptake and transpiration. In stomatal guard cells under blue light, plasma membrane H(+)-ATPase is phosphorylated and activated via blue light-receptor phototropins and a signaling mediator BLUS1, and H(+)-ATPase activation drives stomatal opening. However, details of the signaling between phototropins and H(+)-ATPase remain largely unknown. In this study, through a screening of specific inhibitors for the blue light-dependent H(+)-ATPase phosphorylation in guard cells, we identified a Raf-like protein kinase, BLUE LIGHT-DEPENDENT H(+)-ATPASE PHOSPHORYLATION (BHP). Guard cells in the bhp mutant showed impairments of stomatal opening and H(+)-ATPase phosphorylation in response to blue light. BHP is abundantly expressed in the cytosol of guard cells and interacts with BLUS1 both in vitro and in vivo. Based on these results, BHP is a novel signaling mediator in blue light-dependent stomatal opening, likely downstream of BLUS1.