文章基本信息

标题：Dual origins of the intracellular circadian calcium rhythm in the suprachiasmatic nucleus
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作者：Ryosuke Enoki ; Daisuke Ono ; Shigeru Kuroda 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2017
卷号：7
期号：1
DOI：10.1038/srep41733
语种：English
出版社：Springer Nature
摘要：In mammals, the master circadian clock is located in the suprachiasmatic nucleus (SCN), where most neurons show circadian rhythms of intracellular Ca(2+) levels. However, the origin of these Ca(2+) rhythms remains largely unknown. In this study, we successfully monitored the intracellular circadian Ca(2+) rhythms together with the circadian PER2 and firing rhythms in a single SCN slice ex vivo, which enabled us to explore the origins. The phase relation between the circadian PER2 and Ca(2+) rhythms, but not between the circadian PER2 and firing rhythms, was significantly altered in Cry1/Cry2 double knockout mice, which display a loss of intercellular synchronization in the SCN. In addition, in Cry1/Cry2 double knockout mice, circadian Ca(2+) rhythms were abolished in the dorsolateral SCN, but were maintained in the majority of the ventromedial SCN. These findings indicate that intracellular circadian Ca(2+) rhythms are composed of an exogenous and endogenous component involving PER2 expression.