文章基本信息

标题：A New Tactic for Label-Free Recognition of β-Trophin via Electrochemiluminescent Signalling on an AuNPs Supported Immuno-Interface
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作者：Lijuan Zheng ; Chen Fang ; Jilin Yan 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2017
卷号：7
期号：1
DOI：10.1038/s41598-017-11750-8
语种：English
出版社：Springer Nature
摘要：In this paper, a new strategy is reported for preparing a label-free β-trophin electrochemiluminescent (ECL) immunosensor with good specificity, reproducibility and stability. An aquagel polymer from the hydrolysis of (3-aminopropyl) trimethoxysilane acted as the linker to catch the Au nanoparticles (AuNPs) on the indium-tin oxide (ITO) substrate by a two-step method. The AuNPs play an important role in enhancing ECL and immobilizing the β-trophin antibody. This immunosensor can test for β-trophin using luminol as an ECL probe. The ECL intensity at the resultant sensor, after the direct immuno-interaction, was proportional to the concentration of β-trophin and had a low limit of quantification as 4.2 ng mL(-1). After deep discussions on the ECL mechanism of this immunosensor, we found that its sensitivity is greatly affected by the presence of oxygen and improved under deoxygenation. We believe that this sensor can be used for clinical cases.