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标题：Expression of CXCR4 on T-cell subsets and Plasma IL-17 Concentrations in Patients with Aplastic Anaemia
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作者：Qian Niu ; Qiang Zhou ; Yumei Liu 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2017
卷号：7
期号：1
DOI：10.1038/s41598-017-08699-z
语种：English
出版社：Springer Nature
摘要：Acquired aplastic anaemia (AA) is caused by T-cells migrating to and attacking bone marrow (BM) in response to chemokines (e.g., CXCR4). We investigated CXCR4 expressions on circulating T-cell subsets, plasma IL-17A concentrations, and their correlations with AA manifestations. We enrolled 71 patients with acquired AA (36 severe AA cases [SAA] and 35 non-severe AA cases [NSAA]) and 42 healthy volunteers. We used flow cytometry and ELISA to measure circulating CD4(+) and CD8(+) T-cells, their CXCR4 expressions, and plasma IL-17A concentrations. Compared to the healthy controls, SAA patients had fewer peripheral CD4(+) T-cells, more CD8(+) T-cells, and a significantly decreased CD4(+)/CD8(+) ratio which was positively correlated with AA manifestations. Patients with SAA or NSAA had higher proportions of CD4(+)CXCR4(+) and CD8(+)CXCR4(+) T-cells, which were negatively correlated with haemoglobin concentrations and absolute neutrophil counts. Patients with SAA or NSAA had higher plasma IL-17A concentrations, which were negatively correlated with AA manifestations and the CD4(+)/CD8(+) ratio. IL-17A concentrations showed a very week correlation with CD4(+)CXCR4(+) T-cells frequencies, and no correlation with CD8(+)CXCR4(+) T-cells frequencies. Aberrant CXCR4 expression may allow circulating T-cells, especially CD8(+) T-cells, to infiltrate BM during AA progression. Elevated IL-17A concentrations may contribute to AA progression outside of the CXCR4-SDF-1α axis.