文章基本信息

标题：Structural insights into a 20.8-kDa tegumental-allergen-like (TAL) protein from Clonorchis sinensis
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作者：Chang Hwa Jo ; Jonghyeon Son ; Sulhee Kim 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2017
卷号：7
期号：1
DOI：10.1038/s41598-017-02044-0
语种：English
出版社：Springer Nature
摘要：Survival of Clonorchis sinensis, a cause of human clonorchiasis, requires tegument proteins, which are localized to the tegumental outer surface membrane. These proteins play an important role in a host response and parasite survival. Thus, these proteins are interesting molecular targets for vaccine and drug development. Here, we have determined two crystal structures of the calmodulin like domain (amino acid [aa] positions 1-81) and dynein light chain (DLC)-like domain (aa 83-177) of a 20.8-kDa tegumental-allergen-like protein from Clonorchis sinensis (CsTAL3). The calmodulin like domain has two Ca(2+)-binding sites (named CB1 and CB2), but Ca(2+) binds to only one site, CB1. The DLC-like domain has a dimeric conformation; the interface is formed mainly by hydrogen bonds between the main chain atoms. In addition, we have determined full-length structure of CsTAL3 in solution and showed the conformational change of CsTAL3 induced by Ca(2+) ion binding using small-angle X-ray scattering analysis and molecular dynamics simulations. The Ca(2+)-bound form has a more extended conformation than the Ca(2+)-free from does. These structural and biochemical analyses will advance the understanding of the biology of this liver fluke and may contribute to our understanding of the molecular mechanism of calcium-responsive and tegumental-allergen-like proteins.