首页    期刊浏览 2024年12月06日 星期五
登录注册

文章基本信息

  • 标题:Long-tip high-speed atomic force microscopy for nanometer-scale imaging in live cells
  • 本地全文:下载
  • 作者:Mikihiro Shibata ; Takayuki Uchihashi ; Toshio Ando
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2015
  • 卷号:5
  • 期号:1
  • DOI:10.1038/srep08724
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:Visualization of morphological dynamics of live cells with nanometer resolution under physiological conditions is highly desired, but challenging. It has been demonstrated that high-speed atomic force microscopy is a powerful technique for visualizing dynamics of biomolecules under physiological conditions. However, application of high-speed atomic force microscopy for imaging larger objects such as live mammalian cells has been complicated because of the collision between the cantilever and samples. Here, we demonstrate that attaching an extremely long (~3 μm) and thin (~5 nm) tip by amorphous carbon to the cantilever allows us to image the surface structure of live cells with the spatiotemporal resolution of nanometers and seconds. We demonstrate that long-tip high-speed atomic force microscopy is capable of imaging morphogenesis of filopodia, membrane ruffles, pit formation, and endocytosis in COS-7, HeLa cells and hippocampal neurons.
国家哲学社会科学文献中心版权所有