文章基本信息

标题：Differentiation of oral bacteria in in vitro cultures and human saliva by secondary electrospray ionization – mass spectrometry
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作者：Lukas Bregy ; Annick R. Müggler ; Pablo Martinez-Lozano Sinues 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep15163
语种：English
出版社：Springer Nature
摘要：The detection of bacterial-specific volatile metabolites may be a valuable tool to predict infection. Here we applied a real-time mass spectrometric technique to investigate differences in volatile metabolic profiles of oral bacteria that cause periodontitis. We coupled a secondary electrospray ionization (SESI) source to a commercial high-resolution mass spectrometer to interrogate the headspace from bacterial cultures and human saliva. We identified 120 potential markers characteristic for periodontal pathogens Aggregatibacter actinomycetemcomitans (n = 13), Porphyromonas gingivalis (n = 70), Tanerella forsythia (n = 30) and Treponema denticola (n = 7) in in vitro cultures. In a second proof-of-principle phase, we found 18 ( P. gingivalis , T. forsythia and T. denticola ) of the 120 in vitro compounds in the saliva from a periodontitis patient with confirmed infection with P. gingivalis , T. forsythia and T. denticola with enhanced ion intensity compared to two healthy controls. In conclusion, this method has the ability to identify individual metabolites of microbial pathogens in a complex medium such as saliva.