文章基本信息

标题：Characterizations of individual mouse red blood cells parasitized by Babesia microti using 3-D holographic microscopy
本地全文：下载
作者：HyunJoo Park ; Sung-Hee Hong ; Kyoohyun Kim 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep10827
语种：English
出版社：Springer Nature
摘要：Babesia microti causes “emergency” human babesiosis. However, little is known about the alterations in B. microti invaded red blood cells ( Bm -RBCs) at the individual cell level. Through quantitative phase imaging techniques based on laser interferometry, we present the simultaneous measurements of structural, chemical, and mechanical modifications in individual mouse Bm -RBCs. 3-D refractive index maps of individual RBCs and in situ parasite vacuoles are imaged, from which total contents and concentration of dry mass are also precisely quantified. In addition, we examine the dynamic membrane fluctuation of Bm -RBCs, which provide information on cell membrane deformability.