文章基本信息

标题：Highly selective fluorescent and colorimetric probe for live-cell monitoring of sulphide based on bioorthogonal reaction
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作者：Fang-Jun Huo ; Jin Kang ; Caixia Yin 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep08969
语种：English
出版社：Springer Nature
摘要：H₂S is the third endogenously generated gaseous signaling compound and has also been known to involve a variety of physiological processes. To better understand its physiological and pathological functions, efficient methods for monitoring of H₂S are desired. Azide fluorogenic probes are popular because they can take place bioorthogonal reactions. In this work, by employing a fluorescein derivative as the fluorophore and an azide group as the recognition unit, we reported a new probe 5-azidofluorescein for H₂S with improved sensitivity and selectivety. The probe shows very low background fluorescence in the absence of H₂S. In the presence of H₂S, however, a significant enhancement for excited fluorescence were observed, resulting in a high sensitivity to H₂S in buffered (10 mmol/L HEPES, pH 7.0) aqueous acetonitrile solution (H₂O/CH₃CN = 1:3, v/v) with a detection limit of 0.035 μ mol/L observed, much lower than the previously reported probes. All these features are favorable for direct monitoring of H₂S with satisfactory sensitivity, demonstrating its value of practical application.