文章基本信息

标题：A calmodulin like EF hand protein positively regulates oxalate decarboxylase expression by interacting with E-box elements of the promoter
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作者：Ayushi Kamthan ; Mohan Kamthan ; Avinash Kumar 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep14578
语种：English
出版社：Springer Nature
摘要：Oxalate decarboxylase ( OXDC ) enzyme has immense biotechnological applications due to its ability to decompose anti-nutrient oxalic acid. Flammulina velutipes , an edible wood rotting fungus responds to oxalic acid by induction of OXDC to maintain steady levels of pH and oxalate anions outside the fungal hyphae. Here, we report that upon oxalic acid induction, a calmodulin (CaM) like protein-Fv CaMLP , interacts with the OXDC promoter to regulate its expression. Electrophoretic mobility shift assay showed that FvCamlp specifically binds to two non-canonical E-box elements (AACGTG) in the OXDC promoter. Moreover, substitutions of amino acids in the EF hand motifs resulted in loss of DNA binding ability of FvCamlp. F. velutipes mycelia treated with synthetic siRNAs designed against Fv CaMLP showed significant reduction in Fv CaMLP as well as OXDC transcript pointing towards positive nature of the regulation. Fv CaMLP is different from other known EF hand proteins. It shows sequence similarity to both CaMs and myosin regulatory light chain (Cdc4), but has properties typical of a calmodulin, like binding of ⁴⁵Ca²⁺, heat stability and Ca²⁺ dependent electrophoretic shift. Hence, FvCaMLP can be considered a new addition to the category of unconventional Ca²⁺ binding transcriptional regulators.