文章基本信息

标题：Ablation of CD8α+ dendritic cell mediated cross-presentation does not impact atherosclerosis in hyperlipidemic mice
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作者：Bart Legein ; Edith M. Janssen ; Thomas L. Theelen 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep15414
语种：English
出版社：Springer Nature
摘要：Clinical complications of atherosclerosis are almost exclusively linked to destabilization of the atherosclerotic plaque. Batf3-dependent dendritic cells specialize in cross-presentation of necrotic tissue-derived epitopes to directly activate cytolytic CD8 Tcells. The mature plaque (necrotic, containing dendritic cells and CD8 Tcells) could offer the ideal environment for cross-presentation, resulting in cytotoxic immunity and plaque destabilization. Ldlr ^−/− mice were transplanted with batf3 ^−/− or wt bone marrow and put on a western type diet. Hematopoietic batf3 deficiency sharply decreased CD8α⁺ DC numbers in spleen and lymph nodes (>80%; P batf3 ^−/− chimeras had a 75% reduction in OT-I cross-priming capacity in vivo . Batf3 ^−/− chimeric mice did not show lower Tcell or other leukocyte subset numbers. Despite dampened cross-presentation capacity, batf3 ^−/− chimeras had equal atherosclerosis burden in aortic arch and root. Likewise, batf3 ^−/− chimeras and wt mice revealed no differences in parameters of plaque stability: plaque Tcell infiltration, cell death, collagen composition, and macrophage and vascular smooth muscle cell content were unchanged. These results show that CD8α⁺ DC loss in hyperlipidemic mice profoundly reduces cross-priming ability, nevertheless it does not influence lesion development. Taken together, we clearly demonstrate that CD8α⁺ DC-mediated cross-presentation does not significantly contribute to atherosclerotic plaque formation and stability.