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标题：Identification of H2S3 and H2S produced by 3-mercaptopyruvate sulfurtransferase in the brain
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作者：Yuka Kimura ; Yukiko Toyofuku ; Shin Koike 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep14774
语种：English
出版社：Springer Nature
摘要：Hydrogen polysulfides (H₂S_n) have a higher number of sulfane sulfur atoms than hydrogen sulfide (H₂S), which has various physiological roles. We recently found H₂S_n in the brain. H₂S_n induced some responses previously attributed to H₂S but with much greater potency than H₂S. However, the number of sulfur atoms in H₂S_n and its producing enzyme were unknown. Here, we detected H₂S₃ and H₂S, which were produced from 3-mercaptopyruvate (3 MP) by 3-mercaptopyruvate sulfurtransferase (3MST), in the brain. High performance liquid chromatography with fluorescence detection (LC-FL) and tandem mass spectrometry (LC-MS/MS) analyses showed that H₂S₃ and H₂S were produced from 3 MP in the brain cells of wild-type mice but not 3MST knockout (3MST-KO) mice. Purified recombinant 3MST and lysates of COS cells expressing 3MST produced H₂S₃ from 3 MP, while those expressing defective 3MST mutants did not. H₂S₃ was localized in the cytosol of cells. H₂S₃ was also produced from H₂S by 3MST and rhodanese. H₂S₂ was identified as a minor H₂S_n, and 3 MP did not affect the H₂S₅ level. The present study provides new insights into the physiology of H₂S₃ and H₂S, as well as novel therapeutic targets for diseases in which these molecules are involved.