文章基本信息

标题：Knockdown of the partner protein OsNAR2.1 for high-affinity nitrate transport represses lateral root formation in a nitrate-dependent manner
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作者：Shuangjie Huang ; Si Chen ; Zhihao Liang 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep18192
语种：English
出版社：Springer Nature
摘要：The morphological plasticity of root systems is critical for plant survival, and understanding the mechanisms underlying root adaptation to nitrogen (N) fluctuation is critical for sustainable agriculture; however, the molecular mechanism of N-dependent root growth in rice remains unclear. This study aimed to identify the role of the complementary high-affinity NO₃⁻ transport protein OsNAR2.1 in NO₃⁻-regulated rice root growth. Comparisons with wild-type (WT) plants showed that knockdown of OsNAR2.1 inhibited lateral root (LR) formation under low NO₃⁻ concentrations, but not under low NH₄⁺ concentrations. ¹⁵N-labelling NO₃⁻ supplies (provided at concentrations of 0–10 mM) demonstrated that (i) defects in LR formation in mutants subjected to low external NO₃⁻ concentrations resulted from impaired NO₃⁻ uptake, and (ii) the mutants had significantly fewer LRs than the WT plants when root N contents were similar between genotypes. LR formation in osnar2 . 1 mutants was less sensitive to localised NO₃⁻ supply than LR formation in WT plants, suggesting that OsNAR2.1 may be involved in a NO₃⁻-signalling pathway that controls LR formation. Knockdown of OsNAR2.1 inhibited LR formation by decreasing auxin transport from shoots to roots. Thus, OsNAR2.1 probably functions in both NO₃⁻ uptake and NO₃⁻-signalling.