文章基本信息

标题：Oxygen-induced cell migration and on-line monitoring biomarkers modulation of cervical cancers on a microfluidic system
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作者：Xuexia Lin ; Qiushui Chen ; Wu Liu 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep09643
语种：English
出版社：Springer Nature
摘要：In this work, we report an integrated microfluidic device for cell co-culture under different concentrations of oxygen, in which the secreted protein VEGF₁₆₅ was on-line qualitatively and semi-quantitatively analyzed by functional nucleic acid, hemin, ABTS and peroxide system. This microfluidic platform allowed investigation of various oxygen and distances effect on cell-to-cell communication. Besides, the microfluidic device was used for real-time analysis of VEGF₁₆₅ protein by aptamer-functionalized microchannels. Under 5% O₂ condition, we found that the migration of CaSki cells was faster than the migration of human umbilical vein endothelial cells. However, the migration of CaSki cells was slower than the migration of HUVECs under 15% O₂ condition. Moreover, the shorter intercellular distances, the quicker cells migration. Furthermore, HIF-1α and VEGF₁₆₅ genes, ROS were analyzed, and the results would provide new perspectives for the diagnosis and medical treatment of cervical cancer.