文章基本信息

标题：C-di-GMP regulates Pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth
本地全文：下载
作者：Song Lin Chua ; Krishnakumar Sivakumar ; Morten Rybtke 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep10052
语种：English
出版社：Springer Nature
摘要：Stress response plays an important role on microbial adaptation under hostile environmental conditions. It is generally unclear how the signaling transduction pathway mediates a stress response in planktonic and biofilm modes of microbial communities simultaneously. Here, we showed that metalloid tellurite (TeO₃^2–) exposure induced the intracellular content of the secondary messenger cyclic di-GMP (c-di-GMP) of Pseudomonas aeruginosa . Two diguanylate cyclases (DGCs), SadC and SiaD, were responsible for the increased intracellular content of c-di-GMP. Enhanced c-di-GMP levels by TeO₃^2– further increased P. aeruginosa biofilm formation and resistance to TeO₃^2–. P. aeruginosa Δ sadC Δ siaD and PAO1/p_lac- yhjH mutants with low intracellular c-di-GMP content were more sensitive to TeO₃^2– exposure and had low relative fitness compared to the wild-type PAO1 planktonic and biofilm cultures exposed to TeO₃^2–. Our study provided evidence that c-di-GMP level can play an important role in mediating stress response in microbial communities during both planktonic and biofilm modes of growth.