文章基本信息

标题：Characterization of stem/progenitor cell cycle using murine circumvallate papilla taste bud organoid
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作者：Eitaro Aihara ; Maxime M. Mahe ; Michael A. Schumacher 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep17185
语种：English
出版社：Springer Nature
摘要：Leucine-rich repeat-containing G-protein coupled receptor 5-expressing (Lgr5⁺) cells have been identified as stem/progenitor cells in the circumvallate papillae, and single cultured Lgr5⁺ cells give rise to taste cells. Here we use circumvallate papilla tissue to establish a three-dimensional culture system (taste bud organoids) that develops phenotypic characteristics similar to native tissue, including a multilayered epithelium containing stem/progenitor in the outer layers and taste cells in the inner layers. Furthermore, characterization of the cell cycle of the taste bud progenitor niche reveals striking dynamics of taste bud development and regeneration. Using this taste bud organoid culture system and FUCCI2 transgenic mice, we identify the stem/progenitor cells have at least 5 distinct cell cycle populations by tracking within 24-hour synchronized oscillations of proliferation. Additionally, we demonstrate that stem/progenitor cells have motility to form taste bud organoids. Taste bud organoids provides a system for elucidating mechanisms of taste signaling, disease modeling, and taste tissue regeneration.