文章基本信息

标题：Ultraviolet light triggers the conversion of Cu2+-bound Aβ42 aggregates into cytotoxic species in a copper chelation-independent manner
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作者：Xiongwei Dong ; Zhe Zhang ; Dan Zhao 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep13897
语种：English
出版社：Springer Nature
摘要：Increasing evidence indicates that abnormal Cu²⁺ binding to Aβ peptides are responsible for the formation of soluble Aβ oligomers and ROS that play essential roles in AD pathogenesis. During studying the Cu²⁺-chelating treatment of Cu²⁺-bound Aβ₄₂ aggregates, we found that UV light exposure pronouncedly enhances cytotoxicity of the chelator-treated and -untreated Cu²⁺-bound Aβ₄₂ aggregates. This stimulated us to thoroughly investigate (1) either the chelation treatment or UV light exposure leads to the increased cytotoxicity of the aggregates, and (2) why the chelator-treated and -untreated Cu²⁺-bound Aβ₄₂ aggregates exhibit the increased cytotoxicity following UV light exposure if the latter is the case. The data indicated that the controlled UV exposure induced the dissociation of Cu²⁺-free and -bound Aβ₄₂ aggregates into SDS-stable soluble oligomers and the production of ROS including H₂O₂ in an UV light intensity- and time-dependent, but Cu²⁺ chelation-independent manner. Although we can’t fully understand the meaning of this finding at the current stage, the fact that the UV illuminated Aβ₄₂ aggregates can efficiently kill HeLa cells implies that the aggregates after UV light exposure could be used to decrease the viability of skin cancer cells through skin administration.