文章基本信息

标题：Dental enamel cells express functional SOCE channels
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作者：Meerim K. Nurbaeva ; Miriam Eckstein ; Axel R. Concepcion 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep15803
语种：English
出版社：Springer Nature
摘要：Dental enamel formation requires large quantities of Ca²⁺ yet the mechanisms mediating Ca²⁺ dynamics in enamel cells are unclear. Store-operated Ca²⁺ entry (SOCE) channels are important Ca²⁺ influx mechanisms in many cells. SOCE involves release of Ca²⁺ from intracellular pools followed by Ca²⁺ entry. The best-characterized SOCE channels are the Ca²⁺ release-activated Ca²⁺ (CRAC) channels. As patients with mutations in the CRAC channel genes STIM1 and ORAI1 show abnormal enamel mineralization, we hypothesized that CRAC channels might be an important Ca²⁺ uptake mechanism in enamel cells. Investigating primary murine enamel cells, we found that key components of CRAC channels (ORAI1, ORAI2, ORAI3, STIM1, STIM2) were expressed and most abundant during the maturation stage of enamel development. Furthermore, inositol 1,4,5-trisphosphate receptor (IP₃R) but not ryanodine receptor (RyR) expression was high in enamel cells suggesting that IP₃Rs are the main ER Ca²⁺ release mechanism. Passive depletion of ER Ca²⁺ stores with thapsigargin resulted in a significant raise in [Ca²⁺]_i consistent with SOCE. In cells pre-treated with the CRAC channel blocker Synta-66 Ca²⁺ entry was significantly inhibited. These data demonstrate that enamel cells have SOCE mediated by CRAC channels and implicate them as a mechanism for Ca²⁺ uptake in enamel formation.