文章基本信息

标题：The molecular architecture of dihydropyrindine receptor/L-type Ca2+ channel complex
本地全文：下载
作者：Hongli Hu ; Zhao Wang ; Risheng Wei 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep08370
语种：English
出版社：Springer Nature
摘要：Dihydropyridine receptor (DHPR), an L-type Ca²⁺ channel complex, plays an essential role in muscle contraction, secretion, integration of synaptic input in neurons and synaptic transmission. The molecular architecture of DHPR complex remains elusive. Here we present a 15-Å resolution cryo-electron microscopy structure of the skeletal DHPR/L-type Ca²⁺ channel complex. The DHPR has an asymmetrical main body joined by a hook-like extension. The main body is composed of a “trapezoid” and a “tetrahedroid”. Homologous crystal structure docking and site-specific antibody labelling revealed that the α1 and α2 subunits are located in the “trapezoid” and the β subunit is located in the “tetrahedroid”. This structure revealed the molecular architecture of a eukaryotic Ca²⁺ channel complex. Furthermore, this structure provides structural insights into the key elements of DHPR involved in physical coupling with the RyR/Ca²⁺ release channel and shed light onto the mechanism of excitation-contraction coupling.