文章基本信息

标题：miR-19b regulates hTERT mRNA expression through targeting PITX1 mRNA in melanoma cells
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作者：Takahito Ohira ; Sunamura Naohiro ; Yuji Nakayama 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep08201
语种：English
出版社：Springer Nature
摘要：Human telomerase reverse transcriptase ( hTERT ) plays a crucial role in cancer development. We previously identified paired-like homeodomain1 ( PITX1 ) as an hTERT suppressor gene. However, the underlying mechanisms that are involved in the regulation of PITX1 remain unknown. Here, we report that the microRNA-19b (miR-19b) regulates hTERT expression and cell proliferation through inhibition of PITX1 . Compared with normal melanocyte cells, miR-19b expression was higher in most melanoma cells and was accompanied by downregulation of PITX1 . Moreover, overexpression of miR-19b inhibited PITX1 mRNA translation through a miR-19b binding site within the 3′UTR of the PITX1 mRNA. Our combined findings indicate the participation of miR-19b as a novel upstream effector of hTERT transcription via direct targeting of PITX1 .