文章基本信息

标题：Effects of Carbon Dioxide Aerosols on the Viability of Escherichia coli during Biofilm Dispersal
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作者：Renu Singh ; Ajay K. Monnappa ; Seongkyeol Hong 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：5
期号：1
DOI：10.1038/srep13766
语种：English
出版社：Springer Nature
摘要：A periodic jet of carbon dioxide (CO₂) aerosols is a very quick and effective mechanical technique to remove biofilms from various substrate surfaces. However, the impact of the aerosols on the viability of bacteria during treatment has never been evaluated. In this study, the effects of high-speed CO₂ aerosols, a mixture of solid and gaseous CO₂, on bacteria viability was studied. It was found that when CO₂ aerosols were used to disperse biofilms of Escherichia coli , they led to a significant loss of viability, with approximately 50% of the dispersed bacteria killed in the process. By comparison, 75.6% of the biofilm-associated bacteria were viable when gently dispersed using Proteinase K and DNase I. Indirect proof that the aerosols are damaging the bacteria was found using a recombinant E. coli expressing the cyan fluorescent protein, as nearly half of the fluorescence was found in the supernatant after CO₂ aerosol treatment, while the rest was associated with the bacterial pellet. In comparison, the supernatant fluorescence was only 9% when the enzymes were used to disperse the biofilm. As such, these CO₂ aerosols not only remove biofilm-associated bacteria effectively but also significantly impact their viability by disrupting membrane integrity.