文章基本信息

标题：Hybrid bright-field and hologram imaging of cell dynamics
本地全文：下载
作者：Hyeokjun Byeon ; Jaehyun Lee ; Junsang Doh 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2016
卷号：6
期号：1
DOI：10.1038/srep33750
语种：English
出版社：Springer Nature
摘要：Volumetric observation is essential for understanding the details of complex biological phenomena. In this study, a bright-field microscope, which provides information on a specific 2D plane, and a holographic microscope, which provides information spread over 3D volumes, are integrated to acquire two complementary images simultaneously. The developed system was successfully applied to capture distinct T-cell adhesion dynamics on inflamed endothelial layers, including capture, rolling, crawling, transendothelial migration, and subendothelial migration.