文章基本信息

标题：Calcium ions function as a booster of chromosome condensation
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作者：Rinyaporn Phengchat ; Hideaki Takata ; Kenichi Morii 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2016
卷号：6
期号：1
DOI：10.1038/srep38281
语种：English
出版社：Springer Nature
摘要：Chromosome condensation is essential for the faithful transmission of genetic information to daughter cells during cell division. The depletion of chromosome scaffold proteins does not prevent chromosome condensation despite structural defects. This suggests that other factors contribute to condensation. Here we investigated the contribution of divalent cations, particularly Ca(2+), to chromosome condensation in vitro and in vivo. Ca(2+) depletion caused defects in proper mitotic progression, particularly in chromosome condensation after the breakdown of the nuclear envelope. Fluorescence lifetime imaging microscopy-Förster resonance energy transfer and electron microscopy demonstrated that chromosome condensation is influenced by Ca(2+). Chromosomes had compact globular structures when exposed to Ca(2+) and expanded fibrous structures without Ca(2+). Therefore, we have clearly demonstrated a role for Ca(2+) in the compaction of chromatin fibres.