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  • 标题:1H-detected solid-state NMR of proteins entrapped in bioinspired silica: a new tool for biomaterials characterization
  • 本地全文:下载
  • 作者:Enrico Ravera ; Linda Cerofolini ; Tommaso Martelli
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2016
  • 卷号:6
  • 期号:1
  • DOI:10.1038/srep27851
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:Proton-detection in solid-state NMR, enabled by high magnetic fields (>18 T) and fast magic angle spinning (>50 kHz), allows for the acquisition of traditional (1)H-(15)N experiments on systems that are too big to be observed in solution. Among those, proteins entrapped in a bioinspired silica matrix are an attractive target that is receiving a large share of attention. We demonstrate that (1)H-detected SSNMR provides a novel approach to the rapid assessment of structural integrity in proteins entrapped in bioinspired silica.
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