文章基本信息

标题：One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle
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作者：Wiebke Garrels ; Thirumala R. Talluri ; Ronja Apfelbaum 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2016
卷号：6
期号：1
DOI：10.1038/srep21953
语种：English
出版社：Springer Nature
摘要：Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we established a highly efficient one-step multiplex gene transfer system into the bovine genome.