文章基本信息

标题：φXANES: In vivo imaging of metal-protein coordination environments
本地全文：下载
作者：Simon A. James ; Dominic J. Hare ; Nicole L. Jenkins 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2016
卷号：6
期号：1
DOI：10.1038/srep20350
语种：English
出版社：Springer Nature
摘要：We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (φXANES), allows us to spatially depict metal-protein associations in a native, hydrated state whilst avoiding intrinsic chemical damage from radiation. This method was validated using iron-challenged Caenorhabditis elegans to observe marked alterations in redox environment.