文章基本信息

标题：A highly selective and sensitive near-infrared fluorescent probe for imaging of hydrogen sulphide in living cells and mice
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作者：Ling Zhang ; Xi Emily Zheng ; Fang Zou 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2016
卷号：6
期号：1
DOI：10.1038/srep18868
语种：English
出版社：Springer Nature
摘要：Hydrogen sulphide (H₂S), the third endogenous gaseous signalling molecule, has attracted attention in biochemical research. The selective detection of H₂S in living systems is essential for studying its functions. Fluorescence detection methods have become useful tools to explore the physiological roles of H₂S because of their real-time and non-destructive characteristics. Herein we report a near-infrared fluorescent probe, NIR-HS, capable of tracking H₂S in living organisms. With high sensitivity, good selectivity and low cytotoxicity, NIR-HS was able to recognize both the exogenous and endogenous H₂S in living cells. More importantly, it realized the visualization of endogenous H₂S generated in cells overexpressing cystathionine β-synthase (CBS), one of the enzymes responsible for producing endogenous H₂S. The probe was also successfully applied to detect both the exogenous and endogenous H₂S in living mice. The superior sensing properties of the probe render it a valuable research tool in the H₂S-related medical research.