文章基本信息

标题：Spectrally filtered passive Si photodiode array for on-chip fluorescence imaging of intracellular calcium dynamics
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作者：Zheshun Xiong ; Fuu-Jiun Hwang ; Feng Sun 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2019
卷号：9
期号：1
页码：1-9
DOI：10.1038/s41598-019-45563-8
出版社：Springer Nature
摘要：On-chip fluorescence imaging devices are recognized for their miniaturized and implantable nature that can benefit the study of intracellular dynamics at a variety of settings. However, it is challenging to integrate a spectral filter onto such devices (to block the excitation light) that has similar performance to the state-of-the-art emission filters used in fluorescence microscopes. In this work, we report a 100%-yield, spectrally filtered passive Si photodiode array designed for on-chip fluorescence imaging of intracellular Ca 2+ dynamics. Coated with a spectral filter layer that has a high extinction ratio (>10 3 ), our array features high wavelength selectivity (>10 2 ), high linearity (R 2 > 0.98), and low detection limit (45.1 μW 640/30 nm light). Employing fluorescence microscopy as the reference, we demonstrate that our array can conduct on-chip Ca 2+ imaging in C2C12 cells that were chemically triggered to increase their intracellular Ca 2+ levels. Importantly, our array-level data qualitatively captured the static fluorescence image of the cells and the intracellular Ca 2+ dynamics, both of which are correlated with the microscope-collected data. Our results suggest the possible use of the spectrally filtered array towards a miniaturized on-chip fluorescence imaging device, which may open up new opportunities in tissue-level pharmaceutical screening and fundamental studies on cell networks.