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标题：Effectiveness of Dettol Antiseptic Liquid for Inactivation of Ebola Virus in Suspension
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作者：Todd A. Cutts ; M. Khalid Ijaz ; Raymond W. Nims 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2019
卷号：9
期号：1
页码：1-8
DOI：10.1038/s41598-019-42386-5
出版社：Springer Nature
摘要：The efficacy of Dettol Antiseptic Liquid (DAL) for inactivating Ebola virus (Makona C07 variant) (EBOV/Mak) within an organic load in suspension was evaluated per ASTM E1052-11. Three DAL lots were evaluated at dilutions of 1:10, 1:20, and 1:40, with contact times of 0.5, 1, 5, and 10 min. Approximately 7 log 10 tissue culture infectious dose 50 (TCID 50 ) of EBOV/Mak was exposed to DAL at ambient temperature. Each dilution tested reduced the infectious EBOV/Mak titer by ~5 log 10 within one min. Detectable virus was still present after an 0.5-min exposure, but each DAL dilution caused >4 log 10 reduction within this time. Detection of virus below the limit of detection of the TCID 50 assay was assessed by inoculating flasks of Vero E6 cells with undiluted neutralized sample and evaluating the cultures for cytopathic effect after 14 days incubation. No infectious virus was detected with this non-quantitative method in samples subjected to DAL for 5 or 10 min, regardless of the dilution evaluated. The rapid and substantial inactivation of EBOV/Mak by DAL suggests that use of this hygiene product could help prevent the spread of Ebola virus disease during outbreaks.