文章基本信息

标题：Crosstalk between Second Messengers Predicts the Motility of the Growth Cone
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作者：Takahiko Kobayashi ; Fumiaki Nagase ; Kohji Hotta 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2013
卷号：3
期号：1
DOI：10.1038/srep03118
语种：English
出版社：Springer Nature
摘要：Axon guidance involves multiple second messenger signal transduction pathways. Although each signal transduction pathway has been characterized, only a few studies have examined crosstalk between these cascades. Here, we applied a simultaneous second messenger imaging method to the growth cone and demonstrated correlations between cAMP, cGMP, and Ca²⁺. The levels of cAMP and cGMP in non-stimulated freely extending growth cones showed a negative correlation without delay. Although there was no direct correlation between cAMP and Ca²⁺, examination of cross correlations using small time windows showed frequent switching behavior from negative to positive and vice versa . Furthermore, spatially asymmetric cAMP and cGMP signals in freely deviating growth cones were visualized directly. These results indicate that we succeed in relating second messenger crosstalk to growth cone deviation and extension, and also indicate the possibility of predicting axon guidance from this second messenger crosstalk.