文章基本信息

标题：Sensitive detection of ozone by a practical resorufin-based spectroscopic probe with extremely low background signal
本地全文：下载
作者：Yangyang Zhang ; Wen Shi ; Xiaohua Li 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2013
卷号：3
期号：1
DOI：10.1038/srep02830
语种：English
出版社：Springer Nature
摘要：Ozone (O₃) has attracted much attention because of its key role in human health and disease, and its detection is of great importance for various biochemical studies as well as environmental evaluation. Here we develop a simple and practical spectroscopic off-on O₃ probe based on resorufin and the specific reaction of but-3-enyl with O₃. The probe shows an extremely low background spectroscopic signal, but reacts with O₃ producing a distinct color and fluorescence change. The detection limit of the probe for O₃ is 5.9 nM, which corresponds to an ozone concentration of 0.056 mg m⁻³ in air in this study and is lower than the international ambient air quality standard of 0.1 mg m⁻³. More importantly, the proposed probe is worth popularizing, and its applicability has been successfully demonstrated on both the determination of O₃ in real ambient air samples and the imaging of O₃ in biological cells.