文章基本信息

标题：Customized patterned substrates for highly versatile correlative light-scanning electron microscopy
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作者：Lorena Benedetti ; Elisa Sogne ; Simona Rodighiero 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：4
期号：1
DOI：10.1038/srep07033
语种：English
出版社：Springer Nature
摘要：Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical challenges. We here describe our customized patterned glass substrates, which improve the feasibility of correlative fluorescence/confocal and scanning electron microscopy.