文章基本信息

标题：Development of Amperometric Glucose Biosensor Based on Prussian Blue Functionlized TiO2 Nanotube Arrays
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作者：Zhi-Da Gao ; Yongfang Qu ; Tongtong Li 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：4
期号：1
DOI：10.1038/srep06891
语种：English
出版社：Springer Nature
摘要：Amperometric biosensors consisting of oxidase and peroxidase have attracted great attention because of their wide application. The current work demonstrates a novel approach to construct an enzymatic biosensor based on TiO₂ nanotube arrays (TiNTs) as a supporting electrode on which Prussian Blue (PB)-an “artificial enzyme peroxidase” and enzyme glucose oxidase (GOx) have been immobilized. For this, PB nanocrystals are deposited onto the nanotube wall photocatalytically using the intrinsic photocatalytical property of TiO₂, and the GOx/AuNPs nanobiocomposites are subsequently immobilized into the nanotubes via the electrodeposition of polymer. The resulting electrode exhibits a fast response, wide linear range, and good stability for glucose sensing. The sensitivity of the sensor is as high as 248 mA M⁻¹ cm⁻², and the detection limit is about 3.2 μM. These findings demonstrate a promising strategy to integrate enzymes and TiNTs, which could provide an analytical access to a large group of enzymes for bioelectrochemical applications including biosensors and biofuel cells.