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标题：Wide-field imaging and flow cytometric analysis of cancer cells in blood by fluorescent nanodiamond labeling and time gating
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作者：Yuen Yung Hui ; Long-Jyun Su ; Oliver Yenjyh Chen 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：4
期号：1
DOI：10.1038/srep05574
语种：English
出版社：Springer Nature
摘要：Nanodiamonds containing high density ensembles of negatively charged nitrogen-vacancy (NV⁻) centers are promising fluorescent biomarkers due to their excellent photostability and biocompatibility. The NV⁻ centers in the particles have a fluorescence lifetime of up to 20 ns, which distinctly differs from those ( in vivo by time gating. Here, we demonstrate the feasibility of using fluorescent nanodiamonds (FNDs) as optical labels for wide-field time-gated fluorescence imaging and flow cytometric analysis of cancer cells with a nanosecond intensified charge-coupled device (ICCD) as the detector. The combined technique has allowed us to acquire fluorescence images of FND-labeled HeLa cells in whole blood covered with a chicken breast of ~0.1-mm thickness at the single cell level, and to detect individual FND-labeled HeLa cells in blood flowing through a microfluidic device at a frame rate of 23 Hz, as well as to locate and trace FND-labeled lung cancer cells in the blood vessels of a mouse ear. It opens a new window for real-time imaging and tracking of transplanted cells (such as stem cells) in vivo .