文章基本信息

标题：Monitoring Phospholipase A2 Activity with Gd-encapsulated Phospholipid Liposomes
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作者：Zhiliang Cheng ; Andrew Tsourkas
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：4
期号：1
DOI：10.1038/srep06958
语种：English
出版社：Springer Nature
摘要：To date, numerous analytical methods have been developed to monitor phospholipase A₂ (PLA₂) activity. However, many of these methods require the use of unnatural PLA₂substrates that may alter enzyme kinetics, and probes that cannot be extended to applications in more complex environments. It would be desirable to develop a versatile assay that monitors PLA₂ activity based on interactions with natural phospholipids in complex biological samples. Here, we developed an activatable T1 magnetic resonance (MR) imaging contrast agent to monitor PLA₂ activity. Specifically, the clinically approved gadolinium (Gd)-based MR contrast agent, gadoteridol, was encapsulated within nanometer-sized phospholipid liposomes. The encapsulated Gd exhibited a low T1-weighted signal, due to low membrane permeability. However, when the phospholipids within the liposomal membrane were hydrolyzed by PLA₂, encapsulated Gd was released into bulk solution, resulting in a measureable change in the T1-relaxation time. These activatable MR contrast agents can potentially be used as nanosensors for monitoring of PLA₂ activity in biological samples with minimal sample preparation.