文章基本信息

标题：Increased N-glycosylation of Asn88 in serum pancreatic ribonuclease 1 is a novel diagnostic marker for pancreatic cancer
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作者：Daisuke Nakata
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：4
期号：1
DOI：10.1038/srep06715
语种：English
出版社：Springer Nature
摘要：Alterations of carbohydrate structures in cancer cells are the most promising targets for developing clinical diagnostic reagents. Pancreatic cancer is one of the most difficult cancers to diagnose because it lacks definitive symptoms. Two antibodies were raised against human pancreatic ribonuclease 1 that bind to the enzyme containing unglycosylated Asn⁸⁸, but not when its Asn⁸⁸ is N -glycosylated. Differential studies using these antibodies in immunoassays and Western blot analyses showed a significant increase in the serum levels of pancreatic ribonuclease 1 containing N -glycosylated Asn⁸⁸ in pancreatic cancer patients compared with normal human subjects. Focusing on the increase in an N -glycosylated Asn residue of serum pancreatic ribonuclease 1, specifically Asn⁸⁸, affords a new diagnostic marker for pancreatic cancer. This is the first report of a diagnostic cancer marker that takes advantage of the presence or absence of N -glycosylation at a specific Asn residue of a glycoprotein.