文章基本信息

标题：Mechanosensitive physiology of chlamydomonas reinhardtii under direct membrane distortion
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作者：Seul Ki Min ; Gwang Heum Yoon ; Jung Hyun Joo 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：4
期号：1
DOI：10.1038/srep04675
语种：English
出版社：Springer Nature
摘要：Cellular membrane distortion invokes variations in cellular physiology. However, lack of an appropriate system to control the stress and facilitate molecular analyses has hampered progress of relevant studies. In this study, a microfluidic system that finely manipulates membrane distortion of Chlamydomonas reinhardtii ( C. reinhardtii ) was developed. The device facilitated a first-time demonstration that directs membrane distortion invokes variations in deflagellation, cell cycle, and lipid metabolism. C. reinhardtii showed a prolonged G₁ phase with an extended total cell cycle time, and upregulated Mat3 regulated a cell size and cell cycle. Additionally, increased TAG compensated for the loss of cell mass. Overall, this study suggest that cell biology that requires direct membrane distortion can be realized using this system, and the implication of cell cycle with Mat3 expression of C. reinhardtii was first demonstrated. Finally, membrane distortion can be an attractive inducer for biodiesel production since it is reliable and robust.