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标题：FRET ratiometric probes reveal the chiral-sensitive cysteine-dependent H2S production and regulation in living cells
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作者：Lv Wei ; Long Yi ; Fanbo Song 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：4
期号：1
DOI：10.1038/srep04521
语种：English
出版社：Springer Nature
摘要：Hydrogen sulfide (H₂S) is an endogenously produced gaseous signalling molecule with multiple biological functions. In order to visualize and quantify the endogenous in situ production of H₂S in living cells, here we developed two new sulphide ratiometric probes (SR400 and SR550) based on fluorescence resonance energy transfer (FRET) strategy for live capture of H₂S. The FRET-based probes show excellent selectivity toward H₂S in a high thiol background under physiological buffer. The probe can be used to in situ visualize cysteine-dependent H₂S production in a chiral-sensitive manner in living cells. The ratiometric imaging studies indicated that D-Cys induces more H₂S production than that of L-Cys in mitochondria of human embryonic kidney 293 cells (HEK293). The cysteine mimics propargylglycine (PPG) has also been found to inhibit the cysteine-dependent endogenous H₂S production in a chiral-sensitive manner in living cells. D-PPG inhibited D-Cys-dependent H₂S production more efficiently than L-PPG, while, L-PPG inhibited L-Cys-dependent H₂S production more efficiently than D-PPG. Our bioimaging studies support Kimura's discovery of H₂S production from D-cysteine in mammalian cells and further highlight the potential of D-cysteine and its derivatives as an alternative strategy for classical H₂S-releasing drugs.