文章基本信息

标题：Photoacoustic computed microscopy
本地全文：下载
作者：Lei Yao ; Lei Xi ; Huabei Jiang 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：4
期号：1
DOI：10.1038/srep04960
语种：English
出版社：Springer Nature
摘要：Photoacoustic microscopy (PAM) is emerging as a powerful technique for imaging microvasculature at depths beyond the ~1 mm depth limit associated with confocal microscopy, two-photon microscopy and optical coherence tomography. PAM, however, is currently qualitative in nature and cannot quantitatively measure important functional parameters including oxyhemoglobin (HbO₂), deoxyhemoglobin (HbR), oxygen saturation (sO₂), blood flow (BF) and rate of oxygen metabolism (MRO₂). Here we describe a new photoacoustic microscopic method, termed photoacoustic computed microscopy (PACM) that combines current PAM technique with a model-based inverse reconstruction algorithm. We evaluate the PACM approach using tissue-mimicking phantoms and demonstrate its in vivo imaging ability of quantifying HbO₂, HbR, sO₂, cerebral BF and cerebral MRO₂ at the small vessel level in a rodent model. This new technique provides a unique tool for neuroscience research and for visualizing microvasculature dynamics involved in tumor angiogenesis and in inflammatory joint diseases.