文章基本信息

标题：Background free imaging of single mRNAs in live cells using split fluorescent proteins
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作者：Bin Wu ; Jiahao Chen ; Robert H. Singer 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：4
期号：1
DOI：10.1038/srep03615
语种：English
出版社：Springer Nature
摘要：We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image single reporter mRNAs without background fluorescence.