文章基本信息

标题：Uterine Selection of Human Embryos at Implantation
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作者：Jan J. Brosens ; Madhuri S. Salker ; Gijs Teklenburg 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2015
卷号：4
期号：1
DOI：10.1038/srep03894
语种：English
出版社：Springer Nature
摘要：Human embryos frequently harbor large-scale complex chromosomal errors that impede normal development. Affected embryos may fail to implant although many first breach the endometrial epithelium and embed in the decidualizing stroma before being rejected via mechanisms that are poorly understood. Here we show that developmentally impaired human embryos elicit an endoplasmic stress response in human decidual cells. A stress response was also evident upon in vivo exposure of mouse uteri to culture medium conditioned by low-quality human embryos. By contrast, signals emanating from developmentally competent embryos activated a focused gene network enriched in metabolic enzymes and implantation factors. We further show that trypsin, a serine protease released by pre-implantation embryos, elicits Ca²⁺ signaling in endometrial epithelial cells. Competent human embryos triggered short-lived oscillatory Ca²⁺ fluxes whereas low-quality embryos caused a heightened and prolonged Ca²⁺ response. Thus, distinct positive and negative mechanisms contribute to active selection of human embryos at implantation.