文章基本信息

标题：Architecture of high-affinity unnatural-base DNA aptamers toward pharmaceutical applications
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作者：Ken-ichiro Matsunaga ; Michiko Kimoto ; Charlotte Hanson 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2016
卷号：5
期号：1
DOI：10.1038/srep18478
语种：English
出版社：Springer Nature
摘要：We present a remodeling method for high-affinity unnatural-base DNA aptamers to augment their thermal stability and nuclease resistance, for use as drug candidates targeting specific proteins. Introducing a unique mini-hairpin DNA provides robust stability to unnatural-base DNA aptamers generated by SELEX using genetic alphabet expansion, without reducing their high affinity. By this method, >80% of the remodeled DNA aptamer targeting interferon-γ ( K _D of 33 pM) survived in human serum at 37 °C after 3 days under our experimental conditions, and sustainably inhibited the biological activity of interferon-γ.