文章基本信息

标题：Multiplex targeted mass spectrometry assay for one-shot flavivirus diagnosis
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作者：Sheena Wee ; Asfa Alli-Shaik ; Relus Kek 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2019
卷号：116
期号：14
页码：6754-6759
DOI：10.1073/pnas.1817867116
出版社：The National Academy of Sciences of the United States of America
摘要：Targeted proteomic mass spectrometry is emerging as a salient clinical diagnostic tool to track protein biomarkers. However, its strong analytical properties have not been exploited in the diagnosis and typing of flaviviruses. Here, we report the development of a sensitive and specific single-shot robust assay for flavivirus typing and diagnosis using targeted mass spectrometry technology. Our flavivirus parallel reaction monitoring assay ( fv PRM) has the ability to track secreted flaviviral nonstructural protein 1 (NS1) over a broad diagnostic and typing window with high sensitivity, specificity, extendibility, and multiplexing capability. These features, pivotal and pertinent to efficient response toward flavivirus outbreaks, including newly emerging flavivirus strains, circumvent the limitations of current diagnostic assays. fv PRM thus carries high potential in positioning itself as a forerunner in delivering early and accurate diagnosis for disease management.
关键词：targeted proteomics ; PRM ; dengue ; flavivirus ; diagnostics