文章基本信息

标题：Nontoxic nanopore electroporation for effective intracellular delivery of biological macromolecules
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作者：Yuhong Cao ; Enbo Ma ; Stefano Cestellos-Blanco 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2019
卷号：116
期号：16
页码：7899-7904
DOI：10.1073/pnas.1818553116
出版社：The National Academy of Sciences of the United States of America
摘要：We present a simple nanopore-electroporation (NanoEP) platform for delivery of nucleic acids, functional protein, and Cas9 single-guide RNA ribonucleoproteins into both adherent and suspension cells with up to 80% delivery efficiency and >95% cell viability. Low-voltage electric pulses permeabilize a small area of cell membrane as a cell comes into close contact with the nanopores. The biomolecule cargo is then electrophoretically drawn into the cells through the nanopores. In addition to high-performance delivery with low cell toxicity, the NanoEP system does not require specialized buffers, expensive materials, complicated fabrication processes, or cell manipulation; it simply consists of a generic nanopore-embedded water-filter membrane and a low-voltage square-wave generator. Ultimately, the NanoEP platform offers an effective and flexible method for universal intracellular delivery.
关键词：intracellular delivery ; electroporation ; nanotechnology ; nanopore ; genome engineering